Evaluation on the seperated effect of 13 hemoglobin Variants by Lifotronic HPLC system
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2020-07-19
Professor from the hospital evaluated Lifotronic H9 with thalassemia mode by comparing with Sebia Capillary2 and Gene diagnostic kit on ABI etc., and they found that by using the ion exchange high‐performance liquid chromatography (HPLC) method to elute and identify different types of hemoglobin corresponding to different retention time, it takes only 350 seconds to separate hemoglobin HbA1a, HbA1b, HbF, LA1c, HbA1c, P3, P4, HbA0, HbA2 and common HbE, D, S, and C four categories of variant, forming a series of chromatographic peaks. Then, by calculating and comparing the retention time and area of each peak, the corresponding hemoglobin name and percentage content are automatically obtained.
